Serum withdrawal alters cellular localization of Abs and SNX2. HeLa cells were transfected with HA-Abs. After 24 h, fetal bovine serum (FBS) was withdrawn for 4 h (serum) prior to NE-PER nuclear fractionation while control cells were maintained in 5% FBS for the duration. A: Shows a representative Western immunoblot of the NE-PER fractions probed with anti-HA, HDAC, and GDI antibodies, as indicated. Each lane represents one quarter of the cytoplasmic (C) and nuclear (N) fractions. B: Quantitative analysis of the relative distribution of Abs, HDAC-1, and GDI in the NE-PER fractions indicate a shift in Abs from nuclear to cytoplasmic pool upon serum withdrawal (mean ± SEM; n = 4). The nuclear and cytoplasmic fractions were indicated by the nuclear and cytoplasmic localized HDAC1 and GDI, respectively.