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. Author manuscript; available in PMC: 2010 Oct 25.
Published in final edited form as: J Cell Physiol. 2005 Jul;204(1):210–218. doi: 10.1002/jcp.20285

Fig. 8.

Fig. 8

Expression of GFP-Abs down regulates endogenous SNX2. HeLa transiently transfected with GFP control or GFP-Abs were enriched by fluorescence activated cell sorter (FACS). A: Crude lysate (15 μg) from three experiments were analyzed by Western immunoblot with anti-SNX2 antibodies. The blots were also probed for actin. B: Signal intensities were quantified using ImageQuant™ 5.2 Software (Amersham Biosciences, Piscataway, NJ). SNX2 (shaded bar) and actin (clear bar) values from GFP-Abs expressing cells were expressed as a percentage of the GFP controls. C: Quantitative RT-PCR analysis of HeLa cells stably transfected with GFP or GFP-Abs. SNX2 mRNA values normalized to that of GAPDH (mean ± SEM; n = 3) were from three independent stable lines.