Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The bait and prey plasmids were co-transformed into Y190 tester strain and selected on Trp, Leu drop-out plates. The transformants were tested for β-galactosidase activity on X-gal filter paper and intensity as well as the time of onset used to assess the strength of the interactions.
| PRA1 prey | Rab3A bait | VAMP2 bait |
|---|---|---|
| WT | ++ | ++ |
| PRA1-(1–164) | − | − |
| N70T | − | − |
| Y78A | − | − |
| S76A | +++ | +++ |
| V161A | +++ | +++ |
| H166A | − | + |
