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. Author manuscript; available in PMC: 2011 Nov 3.
Published in final edited form as: Vaccine. 2010 Aug 25;28(47):7556–7562. doi: 10.1016/j.vaccine.2010.08.054

Fig. 4. Antibody responses in Hp91 immunised mice.

Fig. 4

Serum was obtained from immunised mice (5 mice per group) 10 days after the final immunisation and analysed for antibody levels by ELISA. (A) A 1:100 dilution of the serum from immunised mice as indicated, (B) a 1:100 dilution of serum from mice immunised with OVA protein and Hp91 (0 or 250μg in PBS) or (C) a serial dilution of serum from mice immunised with OVA protein and Hp91 (500 μg) in PBS was added to the plates, followed by a peroxidase-conjugated anti-mouse IgG1 antibody. Plates were developed with TMB substrate and absorbance was analysed on a microplate reader. Asterisks, p < 0.001; Student's t-test.