Table 1.
Bacterial strains, plasmids and primers.
| Strain or plasmid |
Genotype or relevant characteristics | Source, Reference or Construction |
|---|---|---|
| Strain | ||
| 14028s |
Salmonella enterica serovar Typhimurium wild type |
ATCC |
| CS051 | 14028s phoP102::Tn10dCm | S. Miller |
| CS735 | 14028s ΔprgH-K | S. Miller |
| KM293 | 14028s Δspi-1::FRTkanFRT | This study |
| MLC162 | 14028s feoB::Tn10 | Y. Xu |
| MLC375 | 14028s ΔpspA:: FRTcatFRT | This study |
| MLC619 | 14028s ΔentC:: FRTkanFRT | (Crouch et al., 2008) |
| MM2165 | SL1344 mntH11::kan | (Kehres et al., 2000) |
| MM2562 | SL1344 sitA::MudCm | (Kehres et al., 2000) |
| SL2926 | 14028s invA::kan | (Galan and Curtiss, 1989) |
| SL3627 | 14028s ssrA::aph | S. Libby |
| SL3628 | 14028s ssrB::aph | S. Libby |
| TF951 | 14028s rpoE::cat | (Testerman et al., 2002) |
| JK224 | 14028s ΔpspA | This study |
| JK339 | 14028s ΔSPI-1:: FRTkanFRT | P22(KM293) × 14028s |
| JK451 | 14028s / pJK645 | This study |
| JK560 | 14028s phoP102::Tn10dCm | P22(CS051) × 14028s |
| JK601 | 14028s mntH11::kan | P22(MM2165) × 14028s |
| JK603 | 14028s sitA::MudCm | P22(MM2562) × 14028s |
| JK610 | 14028s ΔpspA::FRT | This study |
| JK620 | 14028s ΔpspA::FRT/pJK664 | This study |
| JK695 | 14028s feoB::Tn10 | P22(MLC162) × 14028s |
| JK713 | 14028s mntH11::kan feoB::Tn10 ΔzupT::FRT | This study |
| JK715 | 14028s ΔzupT:: FRTkanFRT | This study |
| JK725 | 14028s sitA::MudCm feoB::Tn10 ΔzupT:: FRTkanFRT |
This study |
| JK730 | 14028s mntH11::kan sitA::MudCm feoB::Tn10 ΔzupT::FRT |
This study |
| JK752 | 14028s sitA::MudCm feoB::Tn10 ΔzupT:: FRTkanFRT ΔpspA::FRT |
This study |
| JK753 | 14028s mntH11::kan feoB::Tn10 ΔzupT::FRT ΔpspA::FRT |
This study |
| JK755 | 14028s mntH11::kan sitA:: MudCm feoB::Tn10 ΔzupT::FRT ΔpspA::FRT |
This study |
| JK768 | 14028s/pRB3 | This study |
| JK770 | 14028s mntH11::kan feoB::Tn10 ΔzupT::FRT/pRB3 |
This study |
| JK771 | 14028s sitA:: MudCm feoB::Tn10 ΔzupT:: FRTkanFRT pRB3 |
This study |
| JK774 | 14028s sitA::MudCm feoB::Tn10 ΔzupT:: FRTkanFRT ΔpspA::FRT/pRB3 |
This study |
| JK775 | 14028s sitA::MudCm feoB::Tn10 ΔzupT:: FRTkanFRT ΔpspA::FRT/pJK664 |
This study |
| JK776 | 14028s mntH11::kan feoB::Tn10 ΔzupT::FRT ΔpspA::FRT/pRB3 |
This study |
| JK777 | 14028s mntH11::kan feoB::Tn10 ΔzupT::FRT ΔpspA::FRT/pJK664 |
This study |
| JK778 | 14028s mntH11::kan sitA:: MudCm feoB::Tn10 ΔzupT:: FRT/pRB3 |
This study |
| JK780 | 14028s mntH11::kan sitA::MudCm feoB::Tn10 ΔzupT::FRT ΔpspA::FRT/pRB3 |
This study |
| JK823 | 14028s ΔznuABC:: FRTkanFRT | This study |
| JK841 | 14028s ΔentC::FRT | P22(MLC619) × 14028s |
| JK854 | 14028s ΔentC::FRT mntH11::kan sitA::MudCm feoB::Tn10 ΔzupT::FRT |
This study |
| JK859 | 14028s ΔentC::FRT mntH11::kan sitA::MudCm feoB::Tn10 ΔzupT::FRT ΔpspA::FRT |
This study |
| JK860 | 14028s mntH11::kan sitA::MudCm feoB::Tn10 ΔznuABC::FRT/pRB3 |
This study |
| JK862 | 14028s mntH11::kan sitA::MudCm feoB::Tn10 ΔznuABC::FRT ΔpspA::FRT/pRB3 |
This study |
| JK863 | 14028s mntH11::kan sitA::MudCm feoB::Tn10 ΔznuABC::FRT ΔpspA::FRT/pJK664 |
This study |
| JK865 | 14028s mntH11::kan sitA::MudCm feoB::Tn10 ΔznuABC::FRT ΔzupT::FRT/pRB3 |
This study |
| JK866 | 14028s mntH11::kan sitA::MudCm feoB::Tn10 ΔznuABC::FRT ΔzupT::FRT/pJK671 |
This study |
| JK867 | 14028s mntH11::kan sitA::MudCm feoB::Tn10 ΔznuABC::FRT ΔzupT::FRT ΔpspA::FRT/pRB3 |
This study |
| JK869 | 14028s mntH11::kan sitA::MudCm feoB::Tn10 ΔznuABC::FRT ΔzupT::FRT ΔpspA::FRT/pJK671 |
This study |
| JK878 | 14028s ΔentC::FRT mntH11::kan feoB::Tn10 ΔzupT::FRT |
This study |
| JK879 | 14028s ΔentC::FRT mntH11::kan feoB::Tn10 ΔzupT::FRT ΔpspA::FRT |
This study |
| JK937 | 14028s invA::kan | P22(SL2926) × 14028s |
| Plasmid | ||
| pKD3 | bla FRTcatFRT PS1 PS2 ori6K | (Datsenko and Wanner, 2000) |
| pKD4 | bla FRTkanFRT PS1 PS2 ori6K | (Datsenko and Wanner, 2000) |
| pKD46 | bla araC-ParaB γ β exo oriR101 repA101ts | |
| pCP20 | bla cat cI857 λPr flp PSC101 oriTS | (Datsenko and Wanner, 2000) |
| pRB3 | par RK2 bla stable low copy cloning vector | (Berggren et al., 1995) |
| pJK645 | pBAD24-His8-PspA | This study |
| pJK664 | pRB3::pspA | This study |
| pJK671 | pRB3::zupT | This study |
| Primer | Primer sequence (5’-3’) | |
| JP5 | GGGCGGTACCTCGCCACTTGTTAGTGTT | |
| JP18 | CAGAACATTATGTGAGGATTGAATTATGGG TATTTTTTCTTTAAGACCCACTTTCACATT |
|
| JP21 | TGATGAGCGACGGCGCGTATCGGCGCCGCC ATTGTCATTACTAAGCACTTGTCTCCTG |
|
| JP61 | CAGAACATTATGTGAGGATTGAATTATGGG TATTTTTTCTTAATGACAATGGCGGCGCCGA TACGCGCCGTCGCTCATCA |
|
| JP62 | TGATGAGCGACGGCGCGT | |
| JP143 | CCCCTATCATGAGCCATCATCATCATCATCA TCATCATGGTATTTTTTCTCGTTTTGC |
|
| JP144 | CCCCATAAGCTTATTGATTATCTTGCTTCAT | |
| JP272 | ACCACTTATTCTGACCTTACTGGCGGGCGC CGCCACCTTTGTGTAGGCTGGAGCTGCTTC |
|
| JP273 | CTATCGTCTGCAAAATGACGAGACTGAGCC CCATGATGGACATATGAATATCCTCCTTAG |
|
| JP279 | AACCGGATCCAATCGTTATCGTCCAGCACG | |
| JP280 | AACCAAGCTTAACCGATACCTATCGTCT | |
| JP316 | CGAACGGATGTCCCTCTCGCCACGGCTTCC ACGACCGCTGGTGTAGGCTGGAGCTGCTTC |
|
| JP317 | AAAGCGACGCGCGGTTGCGGCGGGGATAA TCAGCAGCGACCATATGAATATCCTCCTTA G |
|
| MLC165 | CTTGCTTCATTTTGGCTTTCAACTGCGCCAG CTGCTCGCTGATTTCATCATCGGCTTTCAGT GTAGGCTGGAGCTGCTTG |
|
| MLC166 | AGATCCGCAGAAGCTGGTGCGCCTGATGAT TCAGGAGATGGAAGATACGCTGGTGGAGGT CATATGAATATCCTCCTTAG |