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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Microbiol. 2010 Sep 14;78(3):710–719. doi: 10.1111/j.1365-2958.2010.07362.x

Figure 4. Cysteine-modification analysis of S2168 variants.

Figure 4

Cells expressing the S2168 mutant alleles (indicated on top) were harvested and treated with the membrane-impermeant thiol reagent MTSES at the time indicated below each lane, subjected to organic denaturation and delipidation, PEGylated with PEG-maleimide, and analyzed by SDS-PAGE and western blotting, as described in Materials and Methods. The asterisk, “+1 PEG”, and “+2 PEG” indicate the position of the unmodified monomer, the singly-PEGylated and the doubly-PEGylated species, respectively.