A. Growth curve of S2168 alleles. Lysogen carrying prophage λQ21Δ(SRRzRz1)21 and indicated plasmids were induced at t=0 and monitored for growth as A550. Open circles, no plasmid; closed circles, pS2168; open diamonds, pS2168S44N; open squares pS2168S44T; closed squares, pS2168S16C/S44T.
B. Growth curve of S2168ΔTMD1 alleles. Culture growth was monitored as in A, except that each culture carries plasmids pQ and the indicated plasmids: open circles, plasmid vector pRE (Gründling et al., 2001); closed circles, pS2168ΔTMD1; open squares, pS2168ΔTMD1-S44T; closed squares, pS2168ΔTMD1-S44N.