Table 2.

Triggering time of the early lysis mutants.

loc1	allele2	full-length3	Δ(TMD1)4
	wt	0	0
TMD1	A12L	−35
	G14Q	−35
	G14C	−35
	T15C	−35
	A17Q	−35
	G21Q	−35
loop	S32C	NL	−40
TMD2	A37T	+30	−30
	A38T	+50	−20
	S44T	−35	NL
	S44N	−35	−45

¹Localization of each mutant in the topological domains.

²Mutant alleles.

³Effect of each mutation on the triggering time (m) of the full length holin S²¹68 (wt triggers at 50 m). NL, non-lethal.

⁴Effect of each mutation on the triggering time (m) of the truncated holin S²¹68_ΔTMD1 (wt triggers at 50 m). NL, non-lethal.

Adapted from Pang et al (2010) with permission.