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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Microbiol. 2010 Sep 16;78(3):686–700. doi: 10.1111/j.1365-2958.2010.07355.x

Figure 2. Expression of M.EcoRII in wild-type and mutant cells.

Figure 2

Extracts from HK21 (WT) or the indicated HK21 derivatives, with or without M.EcoRII plasmid pR215, were analyzed by Western blotting with polyclonal antibodies to M.EcoRII. The top portion of the gel was excised and stained with Coomassie blue as a total protein loading control. The Western antibody signals and total protein controls were quantitated using an Infrared Imaging System (see Experimental Procedures). The calculated ratio of M.EcoRII to total protein for the four lanes with significant M.EcoRII is expressed as percentage of wild-type levels at the bottom of the figure.