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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Microbiol. 2010 Sep 16;78(3):686–700. doi: 10.1111/j.1365-2958.2010.07355.x

Figure 5. SsrA tagging is induced by aza-C.

Figure 5

HK21 (WT) or the indicated HK21 derivatives were grown for 2.25 generations in the presence of glucose (0.2%) with or without aza-C, and then washed out of the aza-C-containing media into LB with arabinose (0.05%). The presence of the wild-type or C186W M.EcoRII expressing pBAD-derived plasmid is indicated just below the genotype. Extracts were made after one-hour incubation at 37°C, and equal volumes of cell extract were then analyzed by Western blotting with an SsrA tag polyclonal antibody.