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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Microbiol. 2010 Sep 16;78(3):686–700. doi: 10.1111/j.1365-2958.2010.07355.x

Figure 6. Aza-C hypersensitivity is relieved by ssrA-H6 mutant.

Figure 6

Overnight cultures of strain HK22 ssrA::kan derivatives carrying two plasmids were diluted to 4 × 108 cells/ml. The identities of the plasmids are indicated on the left: ssrA+ (pKW11); ssrA-H6 (pKW24); ΔssrA (pKW1); M.EcoRII (pR234); vector (pRK1). Ten-fold serial dilutions were generated across a microtiter plate and 5 µl of each dilution was spotted onto LB plates with no aza-C (left panels), aza-C at 2 µg/ml (middle panel), or aza-C at 5 µg/ml (right panel). Note that the level of sensitivity with this IPTG-inducible M.EcoRII expression plasmid appears somewhat higher than for the other two expression plasmids in previous experiments. Plates were photographed after overnight incubation at 37°C.