Fig. 6.

Perturbing the establishment of polarity in stellate cells disrupts their integration. (A, C and D) Embryos in which a membrane-bound GFP is driven by G447.2 to visualise the stellate cells, stained for GFP (green) and Ct (red, A), aPKC (red, C) or Dlg (red, D). (B and E–G) dominant active aPKC-expressing stellate cells stained for (B) aPKC (green) and Ct (red); (E) Dlg (green) and aPKC (red); (F) aPKC (red); (G) E-Cad (green) and aPKC (red). When aPKCN is expressed in stellate cells they do not move into the tubules as in wild-type (compare A with B, arrows). (C and D) In wild-type tubules the stellate cells move into, and touch the tubule lumen (C, arrows) and adopt a columnar morphology (D, arrow). (E and F) When aPKC is dominantly activated in the stellate cells, they do not integrate into the tubules and are abnormal in shape (E and F arrows). (G) Stellate cells that fail to integrate form basal accumulations of E-Cad (G, arrows). All images are of stage 15 embryos. Arrows point to stellate cells.