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. 2010 Jul 27;1(4):1–10. doi: 10.1038/ncomms1041

Figure 5. TB4 is downregulated in Hand1-null yolk sacs.

Figure 5

Hand1 and TB4 are coexpressed in the developing vasculature of the yolk sac at E8.5 in wild-type (+/+) embryos (a, c) but not in Hand1-null yolk sacs (−/−) (e). TB4 expression is found in the cytoplasm of wild-type embryos as revealed by counterstaining nuclear DNA with bis-benzamide (b, d), but is not detected in Hand1-null yolk sacs (f). TB4 is evident in the remodelling vascular plexus (c; highlighted by white arrowheads) in wild-type yolk sacs but significantly downregulated in Hand1-null yolk sacs (e–g). Images were captured at equivalent light intensity and filter settings and levels of TB4 were detected in Hand1-null yolk sacs as equivalent to background as determined by ImageJ scans of six regions of yolk sac vasculature across two wild-type and two Hand1-null yolk sacs (g). Error bars are s.e.m., where n = mean ImageJ fluorescence across six regions of yolk sac vasculature per genotype. **P ≤ 0.01 (Student's t-test). Scale bar in a, 40 μm (a–f). AU, arbitrary units.