Table 1. Total number of pyrosequencing sequences after quality control and chimera screening.
Samples | Prestwick | LH | Totals | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 1 | |
QC tag reads | 27,206 | 23,877 | 21,042 | 19,031 | 49,476 | 39,054 | 35,794 | 54,227 | 35,995 | 305,702 |
Chimera check reads | 13,758 | 14,774 | 13,534 | 12,856 | 42,741 | 23,932 | 29,641 | 40,234 | 25,751 | 217,221 |
Data are shown for reads passing the initial quality trimming, tag matching and size culling steps (QC tag reads) and reads underpinning OCTUs that were estimated to be non-chimeric (Chimera check reads) from the Prestwick and Littlehampton (LH) sample sites.