Table 1.

Allele Quantification Values at the 132 Position of IDH1

Mutant IDH1 R132H					Wild-type IDH1 R132
(CGT > CAT)					(CGT)
Sample ID	Diagnosis	Sex	Age (years)	% A	Sample ID	Diagnosis	Sex	Age (years)	% A
550	GBM	M	9	23.33 ± 6.69 (n = 3)	546	GBM	F	10	7.66 ± 1.02 (n = 3)
608	AA III	F	16	47.00 ± 4.00 (n = 2)	548	GBM	F	14	12.33 ± 3.84 (n = 3)
640	AA III	M	5	55.00 + 2.57 (n = 3)	549	GBM	F	7	8.33 + 0.66 (n = 3)
730	AA III	M	13	50.0 ± 34.39 (n = 3)	564	GBM	F	17	11.33 ± 1.33 (n = 3)
677	GBM	M	17	34.00 + 10.40 (n = 3)	596	AA III	M	13	9.66 ± 3.48 (n = 3)
726	AA III	M	7	29.33 ± 1.20 (n = 3)	646	GBM	F	14	10.00 ± 3.60 (n = 3)
					719	GBM	M	4	8.66 ± 0.88 (n = 3)
					738	AA III	M	23	9.66 + 0.88 (n = 3)
					751	AA III	M	7	9.66 ± 2.18 (n = 3)
					698	GBM	F	10	15.33 ± 0.66 (n = 3)

IDH1 gene analysis of six pediatric high-grade glioma samples at position 395, resulting in the R132H mutation, along with 10 tumor samples exclusively carrying wild-type alleles. The numerical values represent the percentage of the mutated nucleotide determined by the allele quantification method. Values between the groups were statistically significant at p < 0.001 when compared by one-way ANOVA. M, male; F, female. The wild type and the mutant codon 132 of IDH1 appears in parentheses with the respective wild type and mutant base in bold (left). On the right, the wild type codon 132 is in bold.