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. 2010 Nov;12(6):797–807. doi: 10.2353/jmoldx.2010.090199

Figure 2.

Figure 2

Real-time MSP to amplify methylated sequences (placental) of the EF3 marker. A: Real-time MSP performed on four first trimester CVS-maternal blood DNA pairs [N2 (46,XY), N3 (46,XY), N4 (46,XY), and N1 (46, XY)] after bisulfite conversion. B: Real-time MSP to selectively amplify methylated sequences (fetal) of the EF3 marker from maternal plasma DNA. Reactions were performed on DNA extracted from first-trimester maternal plasma, CVS, and maternal blood cells after bisulfite conversion of DNAs from two pregnancies (N5 and N3). Primer pairs are the same used in A. The quality and the amount of all templates have been checked by amplifying samples with a primer pair specific for both methylated and unmethylated templates (control real-time PCR). Upper panels indicate fluorescence curves, whereas lower panels represent electrophoretic analysis of real-time products. MW marker: molecular weight marker. CVS: chorionic villus sample.