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. 2010 Nov;12(6):797–807. doi: 10.2353/jmoldx.2010.090199

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© 2010 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

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Detection of sex chromosomes/autosomes ratio in CVS of 45,X pregnancies after bisulfite conversion. Real-time MSP of the EF3 marker, normalized with RASSF1A. Reactions were performed on first-trimester CVS samples from four euploid individuals (N6, N7, N8, and N9) and five Turner (45,X) samples (T1, T2, T3, T4, and T5). One euploid individual was used as reference sample in the 2⁻^ΔΔCt calculations, and each of the four euploid samples gave similar results. We reported the graphic results based on the reference euploid sample N8 (fold change = 1, omitted in the figure) that gave the least significant P value. Each Turner sample resulted statistically different from euploid controls (P = 0.0265 for T1, P = 0.0162 for T2, P = 0.0265 for T3, P = 0.0162 for T4, and P = 0.0162 for T5). Gray and white dotted bars ( vs ) indicate, respectively, the average fold changes for euploid and Turner samples (the two groups are significantly different with P = 0.0001).

Inline graphic — Detection of sex chromosomes/autosomes ratio in CVS of 45,X pregnancies after bisulfite conversion. Real-time MSP of the EF3 marker, normalized with RASSF1A. Reactions were performed on first-trimester CVS samples from four euploid individuals (N6, N7, N8, and N9) and five Turner (45,X) samples (T1, T2, T3, T4, and T5). One euploid individual was used as reference sample in the 2⁻^ΔΔCt calculations, and each of the four euploid samples gave similar results. We reported the graphic results based on the reference euploid sample N8 (fold change = 1, omitted in the figure) that gave the least significant P value. Each Turner sample resulted statistically different from euploid controls (P = 0.0265 for T1, P = 0.0162 for T2, P = 0.0265 for T3, P = 0.0162 for T4, and P = 0.0162 for T5). Gray and white dotted bars ( vs ) indicate, respectively, the average fold changes for euploid and Turner samples (the two groups are significantly different with P = 0.0001).