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. 2010 Nov 5;83(5):1146–1155. doi: 10.4269/ajtmh.2010.10-0212

Figure 1.

Figure 1.

Centers for Disease Control and Prevention (CDC) testing algorithm and results interpretation for Japanese encephalitis (JE) classification. aCerebrospinal fluid (CSF) were treated in the same manner with the exception that they were not tested for West Nile virus (WNV). bSpecimens without adequate volume remaining to conduct the plaque-reduction neutralization test (PRNT) were re-tested by Japanese encephalitis virus (JEV) and dengue virus (DENV) IgM antibody capture enzyme-linked immunosorbent assay (MAC ELISA) with the COS-1 antigen or JE suckling mouse brain (SMB) antigen. Those with concordant results were considered JE presumptive positive and classified as JE positive; specimens with discordant results were excluded from the sample set. cSamples with positive IgM results to more than one of the antigens tested (JEV, DEN, and/or WNV) and without virus-specific antibody response by PRNT (titers to more than one of the viruses without a 4-fold or greater difference) were classified as inconclusive and excluded from the evaluation specimen panel.