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. 2010 Nov 5;83(5):1146–1155. doi: 10.4269/ajtmh.2010.10-0212

Table 4.

Endpoint dilutions* of IgM detection on three sera and two CSF

Sample Type CDC Panbio XCyton InBios Panbio Adjusted
1 High serum > 12,800 3,200 6,400 > 12,800 N/A
2 High serum > 12,800 200 1,600 > 12,800 N/A
3 High serum > 12,800 3,200 > 12,800 > 12,800 N/A
4 High CSF > 1,280 40 20 320 20
5 Low CSF 80 10 < 10 < 10 10
*

Endpoints are given as the reciprocal of the highest dilution at which Japanese encephalitis virus (JEV) IgM was detected by the assay from an initial serum dilution of 1:100 diluted 4-fold to 1:12800 and initial cerebrospinal fluid (CSF) dilution of 1:10 diluted 2-fold to 1:1280. Endpoints of > 12,800 (sera) or > 1,280 (CSF) indicate that JEV IgM was detected by the assay to the final dilution of the analysis.

Based on initial Centers for Disease Control and Prevention (CDC) Japanese encephalitis virus (JEV) IgM antibody capture enzyme-linked immunosorbent assay (MAC ELISA) results at 1:400 (sera) and 1:10 (CSF) screening dilutions. High serum or CSF, P/N > 20; low CSF, P/N < 10.