Figure 4.

Inhibition of re-presentation of gp96-chaperoned peptides through MHC I or MHC II molecules. (A) CD4+ T cell clone 24D3 cells (2 x 10⁴) were cultured with Meth A-derived gp96 (50 µg/ml) in the presence of 5 x 10⁵ irradiated syngeneic APCs which had or had not been pre-treated with BFA or CHQ. Inhibitors were used as described in the text. Stimulation of CD4+ T cells was measured by IL-5 release. (B) CD8+ cells (10⁴) specific for L^d/AH1 epitope of CT26 were cultured with 10⁴ RAW264.7 cells in the presence of gp96 (50 µg/ml) or gp96-AH1 19-mer peptide complexes (50 µg/ml). The APCs were either untreated or treated previously with BFA or CHQ. Stimulation of CD8+ T cells was measured by interferon-γ release.