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NY-ESO-1 antigen-specific CD8+ IFN-γ+ and CD4+ IFN-γ+ responses. The IFN-γ gate was set based on a negative control (alone) sample and applied across other samples (+NY-ESO-1). (A) Representative dot plots. (B) Compiled data. (C) MHC class I immunohistochemical staining (clone A4). (D) HLA-DR immunohistochemical staining (clone YE2/36HLK).
