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. 2010 Nov 1;137(21):3675–3685. doi: 10.1242/dev.044560

Fig. 7.

Fig. 7.

Hydroxylated sphingolipids increase survival upon sterol depletion. (A) Final developmental stages reached by lace hypomorphs l(2)k05305 when fed LDM + 6.2 μg/ml cholesterol. (B) Survival of homozygous l(2)k05305 larvae over time when fed LDM + 6.2 μg/ml cholesterol. (C) RT-PCR detecting mRNA for fa2h and Actin5C in tissues from third instar larvae fed YM. (D) Effect of compromised sphingolipid production on larval survival time when on sterol-depleted food. Survival time is defined as the time at which 50% of larvae remain alive. Bars indicate the average difference in survival time of larvae with the indicated genotypes, compared with control siblings. lace l(2)k05305 larvae have reduced serine palmitoyl transferase activity. tub>fa2hi1 and tub>fa2hi2 larvae ubiquitously express distinct fa2h RNAi constructs under the control of tubulin GAL4. elavII>fa2hi1 and elavIII>fa2hi1 larvae induce fa2h1 RNAi in the nervous system using two distinct elavGAL4 drivers. adh>fa2hi1 larvae induce fa2h1 RNAi in the fat body, oenocytes and anterior midgut. lipo>fa2hi1 larvae induce fa2h1 RNAi in the fat body. npc1b>fa2hi1 larvae induce fa2h1 RNAi in the posterior and anterior midgut. Asterisks indicate significant differences from control siblings. **, P≤0.01, *, P≤0.05. (E) The percentage of tub>fa2hi1 larvae or control siblings that either die as pupae or complete adult development when fed YM. (F) The adult weight of YM-fed tub>fa2hi1 males and females and that of control siblings. (G) Mass spectrometric quantification of sphingolipid species present in the hemolymph. Error bars indicate standard deviations.