Fig. 7.

The midline-positive signals SHH and VEGF are not sufficient to signal dorsal aortae fusion. (A-I) Stage 11 electroporated embryos imaged for vascular-specific Qh1 antibody (A,D,G), direct RFP fluorescence (B,E,H) and magnified overlay of Qh1 and RFP (C,F,I). (A-C) Control RFP embryo with unfused dorsal aortae (n=11). (D-F) Shh+Rfp electroporated embryo showing no midline vessels or dorsal aortae fusion (n=8). (G-I) Vegf+Rfp electroporated embryo showing enlarged dorsal aortae but no dorsal aortae fusion (n=9). da, dorsal