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Localization of IGF1R at the αvβ3 integrin adhesion sites requires Sdc1. Sdc1-positive and negative HUVECs were co-stained for αvβ3 integrin (mouse mAb LM609) and IGF1R (chicken anti-IGF1Rα), αvβ3 integrin (mouse mAb LM609) and Sdc1 (rabbit anti-human-S1ED) or Sdc1 (rabbit anti-human-S1ED) and anti-phosphotyrosine (mouse mAb PY20) followed by Alexa-Fluor-488 (green)- and Alexa-Fluor-546 (red)-conjugated secondary antibodies. Scale bar: 50 μm.
