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XY fluorescent in situ hybridization (FISH). Cytospin slides were created from artificial mixtures (male 0.3% and female 99.7%) of whole blood and fresh peripheral blood mononuclear cells (PBMC) samples used in Figure 1 and 2A, and analyzed with XY FISH. Arrow indicates male cell with Y hybridization signal (green) among female cells of two X (red) hybridization signals. This is one representative experiment our of several performed.
