Figure 2.

Dynamic range and adaptation precision. Dynamic range (A, B) and adaptation precision (C, D) for major (A, C) and minor (B, D) receptors. For dynamic range measurements, concentrations were raised in three-fold steps, and the cells were allowed to adapt prior to each subsequent stimulation. The response for each step was calculated relative to the response towards a saturating stimulus (100 μM MeAsp for buffer-adapted cells) and is plotted against the final concentration of ligand. Precision of adaptation was defined as the adapted FRET value in the presence of a given concentration of ambient ligand, normalized to the adapted value of FRET in buffer. In these experiments, duration of adaptation was limited to 45 min. Error bars indicate standard errors.