Figure 1.
Identification of non-chaperone HSF1-target genes that suppress polyQ aggregation. (A) Summary of 29 novel HSF1-target genes, expression of which was increased in RDT1 and RDT2 lines. The expression of 18 genes (62%) was also increased in HeLa/cHSF1 cells (class a), whereas the expression of the other 11 genes (38%) was constant (class b) or decreased (class c). (B) Percentage of cells with polyQ inclusions in MEF cells co-infected with the retrovirus expressing each indicated gene and adenovirus expressing polyQ81-GFP. A retrovirus expressing an active hHSF1 (hHSF1ΔRDT) was used as a positive control. The averages of five experiments with the mean+s.d. are shown. (C) Soluble and insoluble fractions of polyQ81-GFP were analysed by western blotting (lower), and the insoluble polyQ81-GFP was quantified (upper). The averages of four experiments are shown. Error bars shows the mean+s.d. Statistical significance (P-value) was determined with an unpaired t-test.