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. 2010 Sep 10;29(20):3459–3469. doi: 10.1038/emboj.2010.225

Figure 2.

Figure 2

Overexpression of target genes improves aggregate formation in HSF1-null cells. (A) Percentage of cells having polyQ inclusions (left), accumulation of insoluble polyQ protein (middle), and accumulation of insoluble ubiquitylated protein in WT and HSF1-null MEF cells. Error bars show the mean+s.d. (B) mRNA levels of novel HSF1-target genes in HSF1-null MEF cells relative to the levels in WT cells. The averages of three experiments are shown. Error bars show the mean+s.d. (C) Re-expression of NFATc2, PDZK3, CSRP2, or CRYAB in HSF1-null MEF cells restored HSF1 deficiency, such as overexpression of Hsp70-1, Hsp27, or three Hsps containing Hsp70-1, Hsp27, and Hsp40 (Hsp mix). Percentage of cells with polyQ inclusions (left), accumulation of insoluble polyQ protein (middle), and accumulation of insoluble ubiquitylated protein (right) are shown. The averages of three experiments are shown. Error bars show the mean +s.d. Statistical significance (P-value) was determined with an unpaired t-test.