Fig. 6.

Inversion of the d/v axis by ventral microinjection of dominant negative Rab5 S43N leads to selective accumulation of Toll in endosomes on the dorsal signaling center. (A) Toll-GFP–expressing embryo injected ventrally with Rab5S43N under conditions that invert the d/v axis. The arrows show a shift in the distribution and accumulation of Toll at the opposite (dorsal) side of the embryo, where a newly created signaling center is located. pi, postinjection. (B) Dorsal view of another embryo injected as in A. (C) Ventral view of the embryo injected as in A. (D) Dorsal surface view of an embryo injected as in A, showing accumulation of Toll in a compartment with morphology characteristic of Rab5⁺ early endosomes.