Fig. 3.

Mapping the degree to which point mutations alter functional and structural properties on the crystal structure of PYP. The orientation of PYP was chosen to illustrate the packing of the two N-terminal helices to the central β-sheet of PYP. The pCA chromophore and Cys69 are shown in stick representation. Substitutions that significantly alter the six indicated functional properties are color coded according to the effect of substitutions at these sites, with large increases in values in red, small increases in yellow, large decreases in dark blue, and small decreases in light blue.