Table 1.

Quantifying the evolvability of PYP

	λmax		Φfl	τpB	ΔGU	Production
% affected mutants*	20%	7%	23%	52%	51%	74%
% strongly affected mutants*	12%†	7%‡	9%§	20%¶	28%∥	41%§
Range of values observed	439–470 nm	3–8	0.07–0.94%	0.5 s–30 min	7.2–13.9 kcal/mol	13–200%
Value of WT PYP	447 nm	3.0	0.14	0.9 s	12.5 kcal/mol	100%

^*From a total of 125 substituted positions.

^†Altered by at least 3 nm compared to WT PYP.

^‡Altered by at least 1.5 pH units.

^§Altered by at least ± 50%.

^¶Altered by at least a factor 5.

^∥Altered by at least 2 kcal/mol.