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. 2010 Oct 4;107(42):17968–17973. doi: 10.1073/pnas.1007559107

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Fig. 2. — The E. coli genes gstB and gshA provide resistance to bromoacetate. (A) Michaelis–Menten plot of GstB catalyzed conjugation of glutathione to bromoacetate under saturating glutathione. This plot yields a k_cat value of 27 s^-1 a K_m value for bromoacetate of 5 mM and a second-order rate constant of 5.4 × 10³ M^-1 s^-1. (B) Growth of gstB- and gshA- strains on LB-agar supplemented with bromoacetate (0.45 mM), iodoacetate (0.125 mM) or bromoacetamide (0.175 mM) compared to the parental strain (BW25113).