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. 2010 Sep 20;107(42):17992–17997. doi: 10.1073/pnas.1008260107

Fig. 3.

Fig. 3.

(A) Elevated numbers of F4/80+ macrophages in colon, duodenum, and skin of M5076 sarcoma-bearing mice (TST) compared with NC mice as determined by immunoperoxidase staining of paraffin sections. (B) In tumor mass and mucosa-associated lymphoid tissue (MALT), F4/80 expression is often severe, and tumors are infiltrated with CD3+ and CD45+ cells. Nuclei are counterstained with propidium iodide. Magnification, 200×. (C) Mature/activated macrophage staining intensity determined by immunoperoxidase staining for F4/80 in colon, duodenum, and skin of M5076 sarcoma-bearing mice (TST, red) compared with control cohort (NC, black). F4/80 intensity grades were 1, minimal; 2, mild; 3, moderate. Each dot in each group represents data from one animal. F4/80+ cells in lymphoid aggregates were excluded from the analysis. Horizontal bars represent medians. (D) CCL2 levels are higher in serum of WT B6 tumor-bearing mice over corresponding controls, but not in CCL2 KO mice. CCL2 levels are shown relative to the NC cohort (white). TST1, M5076 sarcoma-bearing mice (black); TST2, B16 melanoma-bearing mice (light gray). Error bars are SDs (n = 3–6). (E) The incidences of γ-H2AX foci increase in colon and duodenum of WT B6J tumor-bearing mice (TST1, TST2) compared with control mice (NC, IC), but not in CCL2 KO mice. Error bars are SDs (n = 5). *Statistically significant difference in a TST mice compared with both NC and IC controls (P < 0.05). (F) OCDLs are increased in colon and duodenum of B6J WT but not of CCL2 KO mice. Error bars are SDs (n = 5). *Statistically significant difference in TST mice compared with both NC and IC controls (P < 0.05).