Fig. 7.

Nox4-dependent enhancement of angiogenic response. Immunoblotting for VEGF in (A) cardiomyocytes and (B) culture supernatants of cardiomyocytes overexpressing Nox4 or a β-gal control (Ctl). Actin and Coomassie blue (CB) staining were used to confirm equal protein loading. Mean data are shown below. **P < 0.01; *P < 0.05 for Nox4 hypoxia vs. normoxia; ^##P < 0.01 for Nox4 vs. β-gal; n = 3/group. (C) Mean data showing effects of conditioned media on tube formation. **P < 0.01 vs. β-gal control (Ctl) or vs. Nox4 overexpression + VEGF antibody; n = 4/group.