Table 1. Effects of 9′ LT mutation on the ACh dose-response curve of human α3β4 receptors expressed in HEK293 cells.

Subtype	EC 50 (µM)	Imax (nA)	Hill slope	Potency ratio	Square root of potency ratio	Cube root of potency ratio	n
α3β4	91.1±10.7	6.67±1.63	1.65±0.23	1	-	-	8
α3β4LT	18.6±1.93	0.48±0.14	1.43±0.11	5.13 [4.31–6.08]	2.26 [2.08–2.47]	-	6
α3LTβ4	5.51±0.91	2.21±0.77	1.27±0.04	18.6 [15.1–22.9]	-	2.65 [2.47–2.84]	7

EC ₅₀, I_max and Hill slope are means ± standard error of the mean of separate fits of each dose-response curve to the Hill equation. Potency ratios relative to the wild type curve were estimated from the parallel fits in which the curves were constrained to have equal Hill slopes. The 2-unit likelihood intervals for the ratios are shown in square brackets.