Figure 7. Northern blot hybridization to detect expression of dicer2 mRNA in cultured mosquito cells.

Total RNA from Aag2 or C6/36 cell cultures was fractionated by agarose gel electrophoresis, transferred to a nylon membrane, and hybridized to either an A. albopictus dcr2 probe (left) or an A. aegypti dcr2 probe (right). The biotinylated probes were detected with the BrightStar BioDetect Kit.