Figure 3. Analysis of TCR repertoires using 2D-F-SSCP.

(A) Spot patterns representing TCR repertoires of naive, activated effector CD4⁺ T cells and Foxp3^GFPlo and Foxp3^GFPhi T_reg cells isolated from control and draining lymph nodes and tumors of tumor-bearing TCR^mini-Foxp3^GFP mice. Blue asterisks indicate control spots used to overlay gel images for analysis. Spots representing T cells expressing the same TCR as Ep63K-specific hybridoma #274 are shown in the populations of Foxp3^GFPlo and Foxp3^GFPhi cells in tumors. The number of spots defined on each gel by image analysis software is shown in lower left corner of each gel image. Similarity between gels is shown as percentage of overlapping spots. Gels compared are indicated by arrows. (B) Pair wise comparison of the total number and number of overlapping spots between naive, activated and T_reg CD4⁺ T cell populations isolated from control and draining lymph nodes and tumors of tumor-bearing TCR^mini-Foxp3^GFP mice. Left table includes comparison of cell populations isolated from different anatomical locations, right upper table shows comparisons of cell populations isolated from the same organ and right lower table shows comparisons of different cell populations isolated from different organs.