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. 2010 Oct 26;5(10):e13632. doi: 10.1371/journal.pone.0013632

Figure 1. Characterisation of Cre Recombinase expression in adult SMARKO testes.

Figure 1

A. Approximately 50% of male SMAR were Cre positive (KO), identified by the presence of a band at 100 bp. Control (C) littermates were negative for Cre. B. Deletion of androgen receptor (AR) in the testes was determined using RT-PCR spanning exon 2. Only the larger 765 bp wild-type (WT) AR band was seen in control testes while the smaller 613 bp KO band was seen in ARKO testes. Both bands were identified in SMARKO testes, showing deletion of AR in a proportion of cells. C. Immunohistochemistry for Cre Recombinase (green) and smooth muscle actin (SMA, blue) showed that Cre Recombinase was expressed selectively (arrow) in the smooth muscle cells surrounding the blood vessels in adult SMARKO testes but not in any other testicular cell types or in any cells in control testes (arrowhead).