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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jun 12;1797(10):1705–1715. doi: 10.1016/j.bbabio.2010.06.003

Fig. 5. Grx2 siRNA sensitized H2O2-induced complex I activity loss in HLE-B3 cells.

Fig. 5

HLE-B3 cells were transfected with Grx2 siRNA, scramble siRNA or without Grx2 siRNA (control) for 48 h, and then incubated with or without 200 μM H2O2 for another 24 h. (A) Western blot analysis of Grx2 protein levels. Mitochondrial lysates of each group was subjected to Western blot analysis with indicated antibodies. The relative pixel density of Grx2 over VDAC is depicted in the right panel. (B) Grx2 siRNA sensitized H2O2-induced complex I activity loss in HLE-B3 cells. HLE-B3 cells were transfected with Grx2 siRNA, scramble siRNA or without Grx2 siRNA for 48 h, and then incubated with or without 200 μM H2O2 for another 24 h. Mitochondria fractions were assayed for complex I activity. Values are expressed as means±S.D., n=3 from three separate experiments. *P < 0.05, comparison of the H2O2-treated Grx2 siRNA and control groups.