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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: J Cell Sci. 2010 Sep 7;123(Pt 19):3336–3346. doi: 10.1242/jcs.070938

Figure 8. Silencing LRP1 in HMF fibroblasts inhibits the paracrine stimulation of fibroblast outgrowth induced by secreted pro-cath-D.

Figure 8

HMFs transfected with Luc siRNA or LRP1 siRNA1 were embedded 48 h post-transfection in the presence of a bottom layer of 3Y1-Ad12 cancer cell lines secreting or not pro-cath-D or D231N pro-cath-D (panel a). LRP1β expression was monitored 48 h post-transfection and before the co-culture assays (panel b). Pro-cath-D secretion was analyzed by immunoblotting after co-culturing for 3 days with Luc siRNA or LRP1 siRNA1 transfected HMFs, with 3Y1-Ad12 control or cath-D-transfected cells (panel c). Phase-contrast optical photomicrographs (panel d, top), and p-nitrotetrazolium violet cell staining (panel d, bottom) are shown after culturing for 3 days with HMFs transfected with Luc siRNA. Phase-contrast optical photomicrographs (panel e, top), and p-nitrotetrazolium violet cell staining (panel e, bottom) are shown after culturing for 3 days with HMFs transfected with LRP1 siRNA1. High magnifications of the boxed regions are displayed. Data from one representative experiment out of 3 is shown. *, non-specific contaminant protein. Bars; - - -, 75 μm; –, 750 μm.