FIG. 1.

Interleukin (IL)-27 induces phosphorylation of signal transducers and activators of transcription protein (STAT)-1 and −3 in Huh7.5 cell. Whole cell lysate was prepared from hepatitis C virus (HCV)-infected and -uninfected Huh7.5 cells stimulated with different concentrations of IL-27 (0, 1, 10, and 100 ng/mL) for 15 min. As positive control for Western blot of STAT-1, −2, −3, and −5, whole cell lysate from 1,000 U/mL of interferon (IFN)-stimulated CD4⁺ T cells was used. A positive control of the activation of STAT-4, cell lysate from 40 ng/mL IL-12-stimulated T cells was used. Western blotting analyses were performed using antibody to phosphorylated STAT-1, −2, −3, −4, or −5. For verification of the same protein expression level, antibodies were stripped from the blots, and then the blots were re-probed with antibody to total STAT-1 or −3.