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Effect of reducing agents on purified, solubilized hA2aR. Samples were incubated with 5 mM DTT or 0.5 mM – 10 mM TCEP, separated on 12% SDS-PAGE, and blotted onto nitrocellulose for detection with an anti-His primary antibody. Arrows denote molecular weight marker (40kDa) for MagicMark™Western protein standard (Life Technologies), close to the mobility of the monomeric band of wild-type hA2aR-His10.
