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. Author manuscript; available in PMC: 2011 Nov 2.
Published in final edited form as: Biochemistry. 2010 Nov 2;49(43):9181–9189. doi: 10.1021/bi101155r

Figure 4.

Figure 4

Figure 4

Figure 4

A) Representative thermal unfolding CD spectra obtained for hA2aR-His10, with temperatures increasing from 5°C to 95°C, in 15°C increments, where lighter greys are higher temperatures. B) Tertiary structure changes monitored through intrinsic tryptophan fluorescence where an excitation wavelength of 290 nm was used. Spectra represent temperatures from 11°C to 79°C, in 8°C increments, where lighter greys are higher temperatures. C) Chemical unfolding of hA2aR-His10 as a function of increasing urea, as determined by intrinsic fluorescence intensity changes at 320 nm. Error bars represent the deviation from the average of two or more biological replicates.