Figure 6.

Denaturation of hA₂aR-His₁₀ via urea addition leads to a loss of receptor ligand-binding activity (decrease in receptor-ligand complexes) as measured through radio-ligand binding with ³[H]-CGS-21,680 in the presence (open circles) or absence (closed circles) of the reducing agent TCEP. Error bars depict standard deviation from the average of three replicate samples at each urea concentration.