. Author manuscript; available in PMC: 2011 Nov 2.

Published in final edited form as: Biochemistry. 2010 Nov 2;49(43):9181–9189. doi: 10.1021/bi101155r

Table 1.

Midpoints of thermal unfolding (T_1/2, °C) determined for hA₂aR-His₁₀.

	WT	+TCEP	+CHA	+Theophylline
CD T_1/2 [°C]ⁱ	58.9 ± 0.4	55.9 ± 0.4	61.1 ± 0.6	58.4 ± 0.4
CPM fluorescence T_1/2 [°C]	41.7 ± 0.2	NDⁱⁱ	46.3 ± 0.5	45.6 ± 0.2

ⁱ

T_1/2 = midpoint of thermal unfolding; values were determined using a model for a single transition between two states

ⁱⁱ

not determined: Addition of TCEP to CPM samples resulted in high levels of background fluorescence, preventing determination of thermal unfolding with CPM.