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. 2010 Jun 1;1(3):1–9. doi: 10.1038/ncomms1020

Figure 4. Shh inactivation alters cell cycle gene expression in genital tubercles and limb buds.

Figure 4

(a, b) Quantitative comparison of transcript levels in ShhcreERT2/C and Shh+/c embryos assayed at three time points (indicated in key) using the RT2-Profiler PCR Array (SABiosciences). Tamoxifen injections were administered at E10.5 and E11.5. Changes in transcript level are expressed as percent change relative to control embryos for G/S-phase cell cycle genes (a) and S- and G2/M-phase genes (b). Error bars show ± s.e.m., n = 3 and asterisks denote significant differences for E11.5 injection/E12.5 collection (Cyclin E1, P=0.030; Dp1, P=0.055; Cyclin B1, P=0.019; Cdc25a, P=0.034; Mcm3, P=0.031), E10.5 injection/E12.5 collection (Cyclin A1, P=0.011; E2f1, P=0.013), E11.5 injection/E13.5 collection (Cyclin A1, P=0.0004; Cyclin E1, P=0.027; Dp1, P=0.033; E2f1, P=0.010; Mcm2, P=0.052). (c) Graph shows comparison of transcript levels in limb buds (grey bars) and genital tubercles (black bars) of ShhcreERT2/C relative to Shh+/C embryos. Tamoxifen injections were administered at E10.5 and embryos were collected for qRT–PCR at E12.5. Changes in transcript level are expressed as percent change relative to control embryos. Error bars show ± s.e.m., n = 3 and asterisks denote significant differences in E2f1 (GT, P=0.010; limb, P=0.008), Dp1 (GT, P=0.012; limb, P=0.026), Myc (GT, P=0.034; limb, P=0.039) and Rb1 (GT, P=0.040; limb, P=0.040).