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. 2010 Nov;136(5):497–513. doi: 10.1085/jgp.200910347

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© 2010 Groulx et al.

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Figure 8. — Simulated double FRET responses. Simulated fluorescence traces (semi-logarithmic scale) for donors distributed between both leaflets (A, C, and E) and donors located at a fraction p of the membrane (B, D, and F). (A and B) Concentration of 1,600 acceptors/µm² with a distribution between both leaflets of b/(1 − b) or located at a position p in the bilayer. The red curve indicates b = 0.5 (equal distribution) or p = 0.5 (center of the bilayer), respectively. (C and D) Same as A and B, but with a concentration of 22,300 acceptors/µm2. (E and F) b or p = 0.5, with increasing concentrations x acceptors/µm² (x as indicated).