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. 2010 Oct 25;207(11):2479–2491. doi: 10.1084/jem.20101376

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© 2010 Wang et al.

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Figure 3. — Proinflammatory stimuli induce classical CD4⁺ T_H2 cells to produce IL-17 cytokine. Sorted CCR6⁻CRTH2⁺CD4⁺ T_H2 cells were maintained with homeostatic cytokines IL-7/IL-15 plus anti–IL-4 and anti–IFN-γ mAbs in the presence or absence of the indicated proinflammatory cytokines for 6 d. Expanded cells were restimulated with PMA plus ionomycin for the analysis of intracellular cytokine production (a) or anti-CD3/CD28 for measurement of cytokines in the culture supernatants by ELISA (b) or were used as cDNA templates for the indicated gene expression analysis by real-time PCR (c). Fold differences in gene expression level between cell/treatment groups marked in the horizontal axis are indicated in the left panel. Data represent the mean (±SD) of five experiments (b). Data are from one of three independent experiments (a and c).