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. 2010 Oct 25;207(11):2479–2491. doi: 10.1084/jem.20101376

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© 2010 Wang et al.

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Figure 6. — The induction of IL-17–producing T_H2 cells occurred in the inflamed lung after allergen exposure. 4GET mice (n = 4) were challenged with indicated allergens plus OVA intranasally every other day for a total of six times before sacrifice. Sorted total (a) or GFP⁺ and GFP⁻ subsets (b) of CD4⁺CD62L⁻CD44^hi memory/effector T cells from lung or indicated lymphoid tissues were restimulated with PMA plus ionomycin for the analysis of intracellular cytokine production (a) or with anti-CD3/CD28 mAbs for 24 h before measurement of cytokines in the supernatants by ELISA (b). Data are from one of three independent experiments (a and b). Data represented as the mean (±SD); four mice per group. LLN, lung draining LNs; MLN, intestine mesenteric LNs.