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. 2010 Oct 18;7:90. doi: 10.1186/1742-4690-7-90

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Copyright ©2010 Inagaki et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SIV core stability in vitro. Concentrated SIVmac239 (Wt; lanes 1-3), SIVmac239Gag205E (205E; lanes 4-6), SIVmac239Gag205E312P (205E312P; lanes 7-9), or SIVmac239Gag205E340M (205E340 M; lanes 10-12) was separated into three fractions (top [a], middle [b], and bottom [c]) by ultracentrifugation under gradient sucrose concentrations in the presence of 0.6% Triton X-100. Each fraction was subjected to Western blot analysis to detect SIV CA p27 proteins (A). A representative result from three sets of experiments is shown. The bottom (c) fractions were also subjected to RT assay (B).